Application
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.| Product Name | BRF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRF1, GTF3B, TAF3C, TAFIII90, TFIIIB90, mTFIIIB90, 2510002F24Rik |
| Product description | BRF1 is one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs.1) Strausberg, R.L., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse BRF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BRF1 antibody can be used for detection of BRF1 by ELISA at 1:62500. BRF1 antibody can be used for detection of BRF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BRF1 |
|---|---|
| Alternative Names | BRF1 GTF3B TAF3C TAFIII90 TFIIIB90 mTFIIIB90 2510002F24Rik |
| Molecular Weight(MW) | 22 kDa |
Application
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | BRF1 antibody can be used for detection of BRF1 by ELISA at 1:62500. BRF1 antibody can be used for detection of BRF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
