Application
ELISA analysis of control antigen and antigen using PSMB8 antibody [1A5].Application
ICC/IF analysis of HeLa cells using PSMB8 antibody [1A5] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of colon tissues using PSMB8 antibody [1A5].Application
IHC-P analysis of intima cancer tissues using PSMB8 antibody [1A5].Application
WB analysis of (1) HeLa, (2) MCF-7, (3) A431, (4) RAJI, (5) MOTL4 and (6) PC-12 lysates using PSMB8 antibody [1A5].Application
WB analysis of human PSMB8 (a.a. 1-272) recombinant protein using PSMB8 antibody [1A5].| Product Name | PSMB8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [1A5] to PSMB8The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t |
| Immunogen | The immunogen corresponding to human PSMB8 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A5 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | RING10 D6S216 LMP7 NKJO PSMB5I ALDD JMP D6S216E |
|---|---|
| Target | PSMB8 |
| Molecular Weight(MW) | 30 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PSMB8 antibody [1A5].
Application
ICC/IF analysis of HeLa cells using PSMB8 antibody [1A5] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of colon tissues using PSMB8 antibody [1A5].
Application
IHC-P analysis of intima cancer tissues using PSMB8 antibody [1A5].
Application
WB analysis of (1) HeLa, (2) MCF-7, (3) A431, (4) RAJI, (5) MOTL4 and (6) PC-12 lysates using PSMB8 antibody [1A5].
Application
WB analysis of human PSMB8 (a.a. 1-272) recombinant protein using PSMB8 antibody [1A5].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
