Application
PSMB8 antibody [N2C3] detects PSMB8 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PSMB8 antibody [N2C3] dilution: 1:250.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMB8 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSMB8 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Product Name | PSMB8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PSMB8 (proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7))The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMB8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Japanese medaka, Xenopus laevis, Dog, Pig, Sheep, BovineJapanese medaka (80%), Xenopus laevis (83%), Dog (91%), Pig (94%), Sheep (93%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D6S216 antibody D6S216E antibody LMP7 antibody MGC1491 antibody PSMB5i antibody RING10 antibody PSMB8 antibody proteasome subunit Y2 antibody macropain subunit C13 antibody proteasome subunit beta type-8 antibody really interesting new gene 10 pr |
|---|---|
| Target | PSMB8 |
| Molecular Weight(MW) | 30 kDa (note) (3) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus |
Application
PSMB8 antibody [N2C3] detects PSMB8 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PSMB8 antibody [N2C3] dilution: 1:250.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMB8 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSMB8 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | A431 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
