Application
Immunofluorescence of BRSK2 in Human Brain cells with BRSK2 antibody at 20 μg/mL.Application
Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 μg/mL.Application
Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | BRSK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRSK2 Antibody: SAD1, SADA, STK29, PEN11B, C11orf7, HUSSY-12, Brain-selective kinase 2 |
| Product description | BRSK2 Antibody: BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.1) Manning G, Whyte DB, Martinez R, et al. The protein kinase complement of the human genome. Science2002; 298:1912-34. |
| Immunogen | BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2. The immunogen is located within amino acids 630 - 680 of BRSK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BRSK2 antibody can be used for detection of BRSK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRSK2 |
|---|---|
| Alternative Names | BRSK2 Antibody: SAD1 SADA STK29 PEN11B C11orf7 HUSSY-12 Brain-selective kinase 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of BRSK2 in Human Brain cells with BRSK2 antibody at 20 μg/mL.
Application
Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 μg/mL.
Application
Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | BRSK2 antibody can be used for detection of BRSK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BRSK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BRSK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
