Application
Immunohistochemistry of BRUCE in human small intestine tissue with BRUCE antibody at 5 μg/mL.| Product Name | BRUCE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE |
| Product description | BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90. |
| Immunogen | BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE. The immunogen is located within amino acids 4760 - 4810 of BRUCE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC6 |
|---|---|
| Alternative Names | BRUCE Antibody: BRUCE APOLLON KIAA1289 Baculoviral IAP repeat-containing protein 6 BIR repeat-containing ubiquitin-conjugating enzyme BRUCE |
| Molecular Weight(MW) | 534 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (100%) |
| Tissue Specificity | BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist. |
Application
Immunohistochemistry of BRUCE in human small intestine tissue with BRUCE antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
|---|---|
| Application Notes | BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BRUCE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BRUCE Antibody is supplied in PBS containing 0.02% sodium azide. |
