Application
Western blot analysis of Bst2 in Daudi cell lysate with Bst2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bst2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bst2 Antibody: GREG, Bst-2, CD317, C87040, DAMP-1, 2310015I10Rik, Bone marrow stromal antigen 2, BST-2 |
| Product description | Bst2 Antibody: Bst2 was first identified as a novel terminal B-cell-restricted antigen termed HM1.24 that is expressed on certain bone marrow cells as well as other tissues. The surface expression of Bst2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line, suggesting that this protein is involved in pre-B-cell growth. Later experiments also showed it to be upregulated in several myeloma cells. More recently, Bst2 was identified an inhibitor of retroviral release from human cells whose activity is antagonized by the HIV-1 accessory protein Vpu. While Bst2 caused retention of virions on cell surfaces or endocytosis into Bst2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release. This activity may represent a potential therapeutic strategy for the treatment of HIV/AIDS.1) Goto T, Kennel SJ, Abe M, et al. A novel membrane antigen selectively expressed on terminally differentiated human B cells. Blood1994; 84:1922-30. |
| Immunogen | Bst2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the mouse Bst2. The immunogen is located within amino acids 60 - 110 of Bst2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Bst2 antibody can be used for detection of Bst2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Bst2 |
|---|---|
| Alternative Names | Bst2 Antibody: GREG Bst-2 CD317 C87040 DAMP-1 2310015I10Rik Bone marrow stromal antigen 2 BST-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (83%) |
Application
Western blot analysis of Bst2 in Daudi cell lysate with Bst2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | Bst2 antibody can be used for detection of Bst2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Bst2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bst2 Antibody is supplied in PBS containing 0.02% sodium azide. |
