Application
Western blot analysis of extracts of various cell lines, using PSMC3 antibody.Application
Immunohistochemistry of paraffin-embedded rat Intestine using PSMC3 antibody at dilution of 1:200 (400x lens).Application
Immunofluorescence analysis of U2OS cell using PSMC3 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of MCF-7 cell using PSMC3 antibody.| Product Name | PSMC3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMC3The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcri |
| Immunogen | Recombinant protein of human PSMC3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TBP1 proteasome 26S subunit ATPase 3 |
|---|---|
| Target | PSMC3 |
Application
Western blot analysis of extracts of various cell lines, using PSMC3 antibody.
Application
Immunohistochemistry of paraffin-embedded rat Intestine using PSMC3 antibody at dilution of 1:200 (400x lens).
Application
Immunofluorescence analysis of U2OS cell using PSMC3 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of MCF-7 cell using PSMC3 antibody.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
