Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Product Name | BTF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BTF3, NACB, BTF3a, BTF3b, BETA-NAC |
| Product description | BTF3 forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.1) Oyake, T., et al., (2005) Gene 345 (2), 271-277. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BTF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BTF3 antibody can be used for detection of BTF3 by ELISA at 1:312500. BTF3 antibody can be used for detection of BTF3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BTF3 |
|---|---|
| Alternative Names | BTF3 NACB BTF3a BTF3b BETA-NAC |
| Molecular Weight(MW) | 23 kDa, 18 kDa, 18 kDa, 18 kDa |
| Entrez Gene | 689 |
|---|---|
| Protein Accession | NP_001032726 |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | BTF3 antibody can be used for detection of BTF3 by ELISA at 1:312500. BTF3 antibody can be used for detection of BTF3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BTF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
