Application
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PSMC3Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.Application
Flow cytometric Analysis of Jurkat cells, using anti-PSMC3 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC201790 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMC3 antibody, and then analyzed by flow cytometry.| Product Name | PSMC3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1G4] to PSMC3The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation. |
| Immunogen | Full length human recombinant protein of human PSMC3 (NP_002795) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1G4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC8487 TBP1 PSMC3 |
|---|---|
| Target | PSMC3 |
| Tissue Specificity | is specific for human PSMC3 (NP_002795). |
Application
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PSMC3
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
Application
Flow cytometric Analysis of Jurkat cells, using anti-PSMC3 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC201790 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMC3 antibody, and then analyzed by flow cytometry.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
