Application
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.Application
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.Application
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.| Product Name | Bub1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A |
| Product description | Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.1) Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88. |
| Immunogen | Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 - 90 of Bub1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BUB1 |
|---|---|
| Alternative Names | Bub1 Antibody: BUB1A BUB1L hBUB1 BUB1A |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.
Application
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Bub1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bub1 Antibody is supplied in PBS containing 0.02% sodium azide. |
