Application
Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 μg/mL.Application
Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 μg/mL.| Product Name | Bub3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint protein BUB3 |
| Product description | Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.1) Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev.2000; 14:2277-82. |
| Immunogen | Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3. The immunogen is located within the last 50 amino acids of Bub3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BUB3 |
|---|---|
| Alternative Names | Bub3 Antibody: BUB3L hBUB3 Mitotic checkpoint protein BUB3 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 μg/mL.
Application
Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Bub3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bub3 Antibody is supplied in PBS containing 0.02% sodium azide. |
