Application
Western blot analysis of lysed extracts from A549, HeLa and 3T3 cells using C-Fos Antibody.| Product Name | C Fos Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p55, AP-1, C-FOS, p55 |
| Product description | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.1) Okazaki K., Sagata N. EMBO J. 14:5048-5059 (1995) Zhang Y., Feng X. H., Derynck R. Nature 394:909-913 (1998) Bossis G., Malnou C. E., Farras R., Andermarcher E., Hipskind R., Rodriguez M., Schmidt D., Muller S., Jariel-Encontre I., Piechaczyk M. Mol. Cell. Biol. 25:6964-6979 (2005) |
| Immunogen | C-Fos antibody was raised against a peptide sequence around aa.28~32 ( S-Y-Y-H-S) derived from Human C-Fos. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOS |
|---|---|
| Alternative Names | p55 AP-1 C-FOS p55 |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous level of total C-Fos protein. |
| Entrez Gene | 2353 |
|---|---|
| Protein Accession | NP_005243.1 |
Application
Western blot analysis of lysed extracts from A549, HeLa and 3T3 cells using C-Fos Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
