Application
Western blot analysis of extracts of various cell lines, using PSMC5 antibody.Application
Immunohistochemistry of paraffin-embedded human lung cancer using PSMC5 antibody at dilution of 1:100 (400x lens).Application
Immunofluorescence analysis of MCF7 cell using PSMC5 antibody. Blue: DAPI for nuclear staining.| Product Name | PSMC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMC5The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown t |
| Immunogen | Recombinant protein of human PSMC5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TBP10 S8 SUG1 TRIP1 p45/SUG p45 proteasome (prosome macropain) 26S subunit ATPase 5 SUG-1 |
|---|---|
| Target | PSMC5 |
| Molecular Weight(MW) | 46 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using PSMC5 antibody.
Application
Immunohistochemistry of paraffin-embedded human lung cancer using PSMC5 antibody at dilution of 1:100 (400x lens).
Application
Immunofluorescence analysis of MCF7 cell using PSMC5 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
