Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
C14orf179 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C14orf179 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Intraflagellar transport protein 43 homolog, IFT43, C14orf179 |
| Product description | 1) Levy, D., et al. BMC Med. Genet.SUPPL 1, S3 (2007) : |
| Immunogen | This C14orf179 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-171 amino acids from the Central region of human C14orf179. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFT43 |
|---|---|
| Alternative Names | Intraflagellar transport protein 43 homolog IFT43 C14orf179 |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
C14orf179 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
