Human membralin is unique and does not share significant sequence homology with other human genes, only membralins of other species. The membralin gene contains 11 exons which encode at least two spliced variants in human cancer. The long form of membralin (membralin-1) comprises all 11 exons, encoding a protein of 620-amino acids long and the short form of membralin (membralin-3) contains all exons except for exon 10, encoding a protein of 408 amino acids. Expression of different membralin isoforms depends on tissue type. The long form, membralin-1, is expressed in ovarian and colorectal carcinomas but not in breast or pancreatic carcinomas, which express only the short splice form, membralin-3. Recent studies suggest that membralin is a novel tumor-associated marker in ovarian serous carcinomas1) Andersson, O., et al., (2002) Brain Res. Gene Expr. Patterns 1 (3-4), 205-212.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human C19orf6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
C19orf6 antibody can be used for detection of C19orf6 by ELISA at 1:62500. C19orf6 antibody can be used for detection of C19orf6 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 1 ug/ml.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
C19orf6 antibody can be used for detection of C19orf6 by ELISA at 1:62500. C19orf6 antibody can be used for detection of C19orf6 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store C19orf6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt