Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMD4 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Product Name | PSMD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMD4 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 4)The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (98%), Xenopus laevis (90%), Dog (98%), Pig (100%), Rabbit (96%), Chicken (95%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AF antibody, AF-1 antibody, ASF antibody, MCB1 antibody, Rpn10 antibody, S5A antibody, pUB-R5 antibody, PSMD4 antibody, S5a/antisecretory factor protein antibody, 26S proteasome regulatory subunit S5A antibody, RPN10 homolog antibody, 26S proteasome non-AT |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMD4 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
