Application
Flow cytometric analysisof MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of C1QA antibody in Jurkat cell line lysates (35ug/lane)Application
C1QA Antibody (RB18692) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | C1QA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Complement C1q subcomponent subunit A, C1QA |
| Product description | C1QA is a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on hromosome 1.1) Dardiotis,E., Koutsou,P. J. Neurol. Sci. (2009) In press |
| Immunogen | This C1QA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the C-terminal region of human C1QA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | C1QA |
|---|---|
| Alternative Names | Complement C1q subcomponent subunit A C1QA |
| Molecular Weight(MW) | 26 kDa |
Application
Flow cytometric analysisof MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of C1QA antibody in Jurkat cell line lysates (35ug/lane)
Application
C1QA Antibody (RB18692) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
