Application
Immunofluorescence analysis of methanol-fixed HeLa, using PSMD7 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:500| Product Name | PSMD7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMD7 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 7)The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMD7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (98%), Rat (97%), Zebrafish (90%), Xenopus laevis (96%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MOV34 antibody, P40 antibody, Rpn8 antibody, S12 antibody, PSMD7 antibody, 26S proteasome regulatory subunit S12 antibody, proteasome subunit p40 antibody, Mov34 homolog antibody, Moloney leukemia virus-34 proviral integration antibody, 26S proteasome non- |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PSMD7 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
