The first mRNA transcript isolated for C2orf3 gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene.1) Anthoni, H., (2007) Hum. Mol. Genet. 16 (6), 667-677.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human C2orf3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
C2orf3 antibody can be used for detection of C2orf3 by ELISA at 1:12500. C2orf3 antibody can be used for detection of C2orf3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat cells at 1 ug/ml.
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application Notes
C2orf3 antibody can be used for detection of C2orf3 by ELISA at 1:12500. C2orf3 antibody can be used for detection of C2orf3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store C2orf3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.