Application
WB analysis of extracts from various cells using PSME1 antibody.Application
IHC-P analysis of human esophagus using PSME1 antibody at a dilution of 1:200.Application
IHC-P analysis of rat testis using PSME1 antibody at a dilution of 1:200.Application
ICC/IF analysis of U2OS cells using PSME1 antibody (red) and DAPI (blue).| Product Name | PSME1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSME1The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulat |
| Immunogen | Recombinant protein of human PSME1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | REGalpha , IFI5111 , PA28alpha , PA28A , proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) |
|---|---|
| Target | PSME1 |
| Molecular Weight(MW) | 28 kDa (note) |
Application
WB analysis of extracts from various cells using PSME1 antibody.
Application
IHC-P analysis of human esophagus using PSME1 antibody at a dilution of 1:200.
Application
IHC-P analysis of rat testis using PSME1 antibody at a dilution of 1:200.
Application
ICC/IF analysis of U2OS cells using PSME1 antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
