Application
Western blot analysis of Psoriasin/HID-5 in A) MCF10A and B) MCF7 cell lysate using at 1 ug/ml.Application
Psoriasin/HID5/S100A7 immunocytochemical detection was performed on (A) untreated and (B) LPS-treated (100 ng/ml, 21 hrs) HOPE-fixed human bronchial epithelial cells. (D) Negative control was included omitting the primary antibody.Application
Formalin-fixed, paraffin-embedded human breast tissue stained with HID5 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.Application
Immunohistochemical analysis of Psoriasin/HID-5 expression in formalin-fixed, paraffin-embedded ductal carcinoma in situ. Shown are successive sections of H&E staining a control monoclonal antibody, andApplication
Western blot analysis of Psoriasin/HID-5 in confluent MCF10A cells; antibody used at 1 ug/ml.| Product Name | Psoriasin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [47C1068] to PsoriasinPsoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes. Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ. Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumour types or abnormally proliferative epithelia. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| IHC: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | S100A7 600353 6278 PSOR1 P31151 Psoriasin S100A7c |
|---|
Application
Western blot analysis of Psoriasin/HID-5 in A) MCF10A and B) MCF7 cell lysate using at 1 ug/ml.
Application
Psoriasin/HID5/S100A7 immunocytochemical detection was performed on (A) untreated and (B) LPS-treated (100 ng/ml, 21 hrs) HOPE-fixed human bronchial epithelial cells. (D) Negative control was included omitting the primary antibody.
Application
Formalin-fixed, paraffin-embedded human breast tissue stained with HID5 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.
Application
Immunohistochemical analysis of Psoriasin/HID-5 expression in formalin-fixed, paraffin-embedded ductal carcinoma in situ. Shown are successive sections of H&E staining a control monoclonal antibody, and
Application
Western blot analysis of Psoriasin/HID-5 in confluent MCF10A cells; antibody used at 1 ug/ml.| Application Notes | IHC: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
