Application
Western blot analysis of CA14 using rabbit polyclonal CA14 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.Application
Flow cytometric analysis of A375 cells using CA14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)| Product Name | CA14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.1) Parkkila,S. Natl. Acad. Sci. U.S.A. 98 (4), 1918-1923 (2001) |
| Immunogen | This CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human CA14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CA14 |
|---|---|
| Alternative Names | Carbonic anhydrase 14 Carbonate dehydratase XIV Carbonic anhydrase XIV CA-XIV CA14 |
| Molecular Weight(MW) | 38 kDa |
Application
Western blot analysis of CA14 using rabbit polyclonal CA14 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.
Application
Flow cytometric analysis of A375 cells using CA14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
