Application
Immunocytochemistry of CaBP7 in HeLa cells with CaBP7 antibody at 4 μg/mL.Application
Immunofluorescence of CABP7 in HeLa cells with CAPB7 antibody at 20 μg/mL.Application
Western blot analysis of CaBP7 in Hela cell lysate with CaBP7 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CaBP7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CaBP7 Antibody: Calcium binding proteins (CaBP) play a crucial role in the calcium-mediated cellular signal transduction pathway in the central nervous system. The CaBP family shares much similarity with CaM I (calmodulin), and it has been shown that CaBP proteins can substitute functionally for, and possibly augment the function of, CaM I. CaBP7 (Calcium-binding protein 7), contains two EF-hand domains for calcium binding and shares 70% homology with CaBP8 and 50% homology with CaM I. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. CaBP7 and 8 possess a targeting mechanism that is unique amongst the CaBPs that may contribute to differential functional Ca2+-sensing by these family members.1) Sokal I, Li N, Verlinde CL, et al. Ca2+-binding proteins in the retina: from discovery to etiology of human disease. Biochim. Biophys. Acta.2000; 1498: 233-51. |
| Immunogen | CaBP7 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CaBP7. The immunogen is located within the first 50 amino acids of CaBP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| CaBP7 antibody can be used for detection of CaBP7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CABP7 |
|---|---|
| Alternative Names | CaBP7 Antibody: CALN2 CALN2 Calcium-binding protein 7 Calneuron II CaBP7 |
Application
Immunocytochemistry of CaBP7 in HeLa cells with CaBP7 antibody at 4 μg/mL.
Application
Immunofluorescence of CABP7 in HeLa cells with CAPB7 antibody at 20 μg/mL.
Application
Western blot analysis of CaBP7 in Hela cell lysate with CaBP7 antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | CaBP7 antibody can be used for detection of CaBP7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CaBP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CaBP7 Antibody is supplied in PBS containing 0.02% sodium azide. |
