Application
CACNA2D2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Fluorescent image of U251 cell stained with CACNA2D2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (Application
Western Blot at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.| Product Name | CACNA2D2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.1) Donato, R., et al. J. Neurosci. 26(48):12576-12586(2006) Ivanov, S.V., et al. Am. J. Pathol. 165(3):1007-1018(2004) Carboni, G.L., et al. Oncogene 22(4):615-626(2003) Honorio, S., et al. Mol. Cell. Probes 15(6):391-393(2001) Angeloni, D., et al. Mol. Cell. Probes 15(2):125-127(2001) |
| Immunogen | This CACNA2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-671 amino acids from the Central region of human CACNA2D2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CACNA2D2 |
|---|---|
| Alternative Names | Voltage-dependent calcium channel subunit alpha-2/delta-2 Voltage-gated calcium channel subunit alpha-2/delta-2 Voltage-dependent calcium channel subunit alpha-2-2 Voltage-dependent calcium channel subunit delta-2 CACNA2D2 KIAA0558 |
| Molecular Weight(MW) | 130 kDa |
Application
CACNA2D2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Fluorescent image of U251 cell stained with CACNA2D2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (
Application
Western Blot at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
