Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CAD (phospho Thr456) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.1) Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996). |
| Immunogen | CAD (phospho Thr456) antibody was raised against a peptide sequence around phosphorylation site of threonine 456 (P-I-T (p)-P-H) derived from Human CAD. |
| Modification | p-Thr456 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAD |
|---|---|
| Alternative Names | PYR1 CAD protein EC 2.1.3.2 EC 3.5.2.3 |
| Molecular Weight(MW) | 242 kDa |
| Entrez Gene | 790 |
|---|---|
| Protein Accession | NP_004332.2 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
