Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2alpha/beta/delta (Phospho-Thr305) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | CaMK2 alpha/beta/delta (phospho Thr305) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.1) Carl W. Tong, J. Physiol., Aug 2004; 558: 927 - 941. |
| Immunogen | CaMK2 alpha/beta/delta (phospho Thr305) antibody was raised against a peptide sequence around phosphorylation site of threonine 305 (I-L-T (p)-T-M) derived from Human CaMK2alpha/beta/delta. |
| Modification | p-Thr305 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK2B, CAMK2D |
|---|---|
| Alternative Names | CAMK2A KCC2A kinase CaMK2-alpha CaMKII-alpha |
| Molecular Weight(MW) | 54 kDa |
| Entrez Gene | 816/817 |
|---|---|
| Protein Accession | NP_741960.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2alpha/beta/delta (Phospho-Thr305) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
