CANT1 belongs to the apyrase family.It is a calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. The enzyme has very low activity towards ADP and even lower activity towards ATP. And it does not hydrolyze AMP and GMP.1) Hermans, K.G., (2008) Cancer Res. 68 (9), 3094-3098.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CANT1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CANT1 antibody can be used for detection of CANT1 by ELISA at 1:12500. CANT1 antibody can be used for detection of CANT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Application Notes
CANT1 antibody can be used for detection of CANT1 by ELISA at 1:12500. CANT1 antibody can be used for detection of CANT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CANT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.