Application
Antibody used in WB on Human HT1080 at 0.2-1 ug/ml.| Product Name | CAPN10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CAPN10 is the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene.1) Kifagi, C., (2008) Diabetes Metab. 34 (3), 273-278. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CAPN10. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CAPN10 antibody can be used for detection of CAPN10 by ELISA at 1:1562500. CAPN10 antibody can be used for detection of CAPN10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAPN10 |
|---|---|
| Alternative Names | CAPN10 CANP10 NIDDM1 |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HT1080 at 0.2-1 ug/ml.| Application Notes | CAPN10 antibody can be used for detection of CAPN10 by ELISA at 1:1562500. CAPN10 antibody can be used for detection of CAPN10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CAPN10 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
