Application
Immunohistochemistry of CAPN6 in human lung tissue with CAPN6 antibody at 2.5 μg/mL.Application
Immunofluorescence of CAPN6 in Human Lung cells with CAPN6 antibody at 20 μg/mL.Application
Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CAPN6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CAPN6 Antibody: Calpains make up a ubiquitously expressed, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 (CAPN6) is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin-like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.1) Croall DE and Ersfeld K. The calpains: modular designs and functional diversity. Genome Biol.2007; 8:216. |
| Immunogen | CAPN6 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human CAPN6. The immunogen is located within the last 50 amino acids of CAPN6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAPN6 |
|---|---|
| Alternative Names | CAPN6 Antibody: CANPX CAPNX CalpM DJ914P14.1 CALPM CANPX Calpain-6 Calpain-like protease X-linked |
Application
Immunohistochemistry of CAPN6 in human lung tissue with CAPN6 antibody at 2.5 μg/mL.
Application
Immunofluorescence of CAPN6 in Human Lung cells with CAPN6 antibody at 20 μg/mL.
Application
Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/mL.| Application Notes | CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CAPN6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CAPN6 Antibody is supplied in PBS containing 0.02% sodium azide. |
