Application
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.Application
Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.| Product Name | CAPS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CAPS1 Antibody: CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4, 5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion.1) Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell1992; 70:765-775. |
| Immunogen | CAPS1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human CAPS1. The immunogen is located within the last 50 amino acids of CAPS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CAPS1 antibody can be used for detection of CAPS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CADPS |
|---|---|
| Alternative Names | CAPS1 Antibody: CAPS CAPS1 CADPS1 CAPS KIAA1121 Calcium-dependent secretion activator 1 Calcium-dependent activator protein for secretion 1 CAPS-1 |
Application
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
Application
Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.| Application Notes | CAPS1 antibody can be used for detection of CAPS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CAPS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CAPS1 Antibody is supplied in PBS containing 0.02% sodium azide. |
