Application
Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody diluted at 1:1000.| Product Name | PTEN (phospho Ser380) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTEN (phospho Ser380) (phosphatase and tensin homolog)This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PTEN (phospho Ser380). The exact sequence is proprietary. |
| Modification | p-Ser380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 47 kDa (note) (3) |
|---|
Application
Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
