Application
Western blot analysis in WiDr cell line lysates (35ug/lane).Application
Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35ug/lane)Application
Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.Application
Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Product Name | CARD6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARD6 is a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.1) Kim,S.S., et.al., Pathology 42 (1), 50-53 (2010) |
| Immunogen | This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARD6 |
|---|---|
| Alternative Names | Caspase recruitment domain-containing protein 6 CARD6 |
| Molecular Weight(MW) | 116 kDa |
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).
Application
Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Application
Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.
Application
Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
