Application
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 μg/mL.Application
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/mL.Application
Western blot analysis of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 μg /ml.| Product Name | CARD8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.1) Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506. |
| Immunogen | CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| CARD8 antibody can be used for detection of CARD8 by Western blot at 2 and 4 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARD8 |
|---|---|
| Alternative Names | CARD8 Antibody: NDPP DACAR DAKAR NDPP1 TUCAN CARDINAL KIAA0955 Caspase recruitment domain-containing protein 8 Apoptotic protein NDPP1 |
Application
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 μg/mL.
Application
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/mL.
Application
Western blot analysis of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 μg /ml.| Application Notes | CARD8 antibody can be used for detection of CARD8 by Western blot at 2 and 4 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CARD8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CARD8 Antibody is supplied in PBS containing 0.02% sodium azide. |
