Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Product Name | CARF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.1) Hasan, M.K., (2002) J. Biol. Chem. 277 (40), 37765-37770. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| CARF antibody can be used for detection of CARF by ELISA at 1:312500. CARF antibody can be used for detection of CARF by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDKN2AIP |
|---|---|
| Alternative Names | CARF CARF |
| Molecular Weight(MW) | 64 kDa |
Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Application Notes | CARF antibody can be used for detection of CARF by ELISA at 1:312500. CARF antibody can be used for detection of CARF by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CARF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
