Application
Immunofluorescence of CARMA1 in Mouse Spleen cells with CARMA1 antibody at 20 μg/mL.Application
Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.Application
Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 μg /ml (lane C), respectively.| Product Name | CARMA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARMA1 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.1) Fanning AS and Anderson JM. Protein modules as organizers of membrane structure.Curr. Opin. Cell Biol. 1999; 11:432-9. |
| Immunogen | CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1. The immunogen is located within the last 50 amino acids of CARMA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 μg/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARD11 |
|---|---|
| Alternative Names | CARMA1 Antibody: PPBL BENTA BIMP3 IMD11 CARMA1 Caspase recruitment domain-containing protein 11 CARD-containing MAGUK protein 1 Carma 1 |
Application
Immunofluorescence of CARMA1 in Mouse Spleen cells with CARMA1 antibody at 20 μg/mL.
Application
Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.
Application
Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 μg /ml (lane C), respectively.| Application Notes | CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 μg/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | CARMA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
