Application
Western Blot at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CARS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable cysteine--tRNA ligase, mitochondrial, Cysteinyl-tRNA synthetase, CysRS, CARS2 |
| Product description | CARS is a class 2 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. It catalyzes the chemical reaction: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).1) Bonnefond L., Biochemistry 44:4805-4816(2005) |
| Immunogen | This CARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-564 amino acids from the C-terminal region of human CARS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CARS2 |
|---|---|
| Alternative Names | Probable cysteine--tRNA ligase mitochondrial Cysteinyl-tRNA synthetase CysRS CARS2 |
| Molecular Weight(MW) | 62 kDa |
Application
Western Blot at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
