Application
Western blot analysis of CASKIN1 in HeLa cell lysate with CLAUDIN4 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | CASKIN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASKIN1 Antibody: ANKS5A, KIAA1306, Caskin-1, CASK-interacting protein 1 |
| Product description | CASKIN1 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting proteins) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN1 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. CASKIN1 coassembles with CASK on the cytoplasmic tail of neurexin 1, suggesting that CASKIN and CASK coat the cytoplasmic tails of neurexins and other cell-surface proteins.1) Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13. |
| Immunogen | CASKIN1 antibody was raised against a 17 amino acid synthetic peptide near the center of human CASKIN1. The immunogen is located within amino acids 720 - 770 of CASKIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CASKIN1 antibody can be used for detection of CASKIN1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASKIN1 |
|---|---|
| Alternative Names | CASKIN1 Antibody: ANKS5A KIAA1306 Caskin-1 CASK-interacting protein 1 |
| Molecular Weight(MW) | Predicted: 157 kDa Observed: 160 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (71%) |
| Tissue Specificity | CASKIN1 antibody is predicted to not cross-react with other CASKIN family members. |
Application
Western blot analysis of CASKIN1 in HeLa cell lysate with CLAUDIN4 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | CASKIN1 antibody can be used for detection of CASKIN1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CASKIN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CASKIN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
