Application Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
Application Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.
Application Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
| Product Name | CASKIN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CASKIN2 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein ) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.1) Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13 |
| Immunogen | CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2. The immunogen is located within amino acids 380 - 430 of CASKIN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASKIN2 |
|---|---|
| Alternative Names | CASKIN2 Antibody: ANKS5B KIAA1139 Caskin-2 CASK-interacting protein 2 |
| Molecular Weight(MW) | Predicted: 123, 132 kDa Observed: 120 kDa |
Application Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
Application Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.
Application Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
| Application Notes | CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CASKIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
