Application
Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.Application
Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.| Product Name | Caspase-12 Antibody (Small) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.1) Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103. |
| Immunogen | Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12. The immunogen is located within the last 50 amino acids of Caspase-12 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: WB:1:500~1:2000 IHC-P:1:50~1:200 |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Casp12 |
|---|---|
| Alternative Names | Caspase-12 Antibody (Small): Caspase-12 CASP-12 |
| Molecular Weight(MW) | Predicted: 46 kDa (uncleaved) Observed: 38 kDa |
Application
Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.
Application
Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.| Application Notes | Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: WB:1:500~1:2000 IHC-P:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-12 antibody (Small) can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small) |
