Application
Staining of formailin fixed paraffin processed mouse liver with anti-Caspase 12 at 2ug/mlApplication
Western blot analysis of Caspase-12 in mouse brain tissue lysate in the absence (A) or presence (B) of blocking peptide with Caspase-12 antibody at 1μg/ml.| Product Name | Caspase 12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 12Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1). |
| Immunogen | Synthetic peptide - amino acids 100-116 of Mouse Caspase 12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Casp12 Caspase12 CASP12P1 Q6UXS9 caspase-12 12364 608633 120329 Caspase 12 |
|---|---|
| Target | Caspase 12 |
Application
Staining of formailin fixed paraffin processed mouse liver with anti-Caspase 12 at 2ug/ml
Application
Western blot analysis of Caspase-12 in mouse brain tissue lysate in the absence (A) or presence (B) of blocking peptide with Caspase-12 antibody at 1μg/ml.| Positive Control | Mouse brain tissue. |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
