Application
Immunohistochemistry of caspase-14 in human liver tissue with caspase-14 antibody at 2.5 μg/mL.Application
Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.| Product Name | Caspase-14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-14 Antibody: Caspases are a family of cysteine proteases that can be divided into apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-14 is highly expressed in embryonic but not adult tissues. It is processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in the keratinocyte terminal differentiation, which is important for the formation of the skin barrier.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human caspase-14. The immunogen is located within the last 50 amino acids of Caspase-14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Casp14 |
|---|---|
| Alternative Names | Caspase-14 Antibody: MICE mini-ICE Caspase-14 Mini-ICE CASP-14 |
| Molecular Weight(MW) | Predicted: 28 kDa Observed: 27 kDa |
Application
Immunohistochemistry of caspase-14 in human liver tissue with caspase-14 antibody at 2.5 μg/mL.
Application
Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.| Application Notes | Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-14 Antibody is supplied in PBS containing 0.02% sodium azide. |
