Application
Immunocytochemistry of caspase-6 in MCF7 with caspase-6 antibody at 1 μg/mL.Application
Western blot analysis of Caspase-6 in MCF7 cell lysate with Caspase-6 antibody (IN) at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of Caspase 6 in MCF7 cells with Caspase 6 antibody at 10 μg/mL.| Product Name | Caspase-6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-6 Antibody: MCH2, MCH2, Caspase-6, Apoptotic protease Mch-2, CASP-6 |
| Product description | Caspase-6 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-6 is an executioner caspase that was identified based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3. It possesses two isoforms, of which only the longer form possesses protease activity. Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-6 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Caspase-6. The immunogen is located within amino acids 160 - 210 of Caspase-6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Casp-6 antibody can be used for the detection of Caspase-6 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP6 |
|---|---|
| Alternative Names | Caspase-6 Antibody: MCH2 MCH2 Caspase-6 Apoptotic protease Mch-2 CASP-6 |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
| Tissue Specificity | Depending on cell lines or tissues used, either full-length or other cleavage products may be observed. |
Application
Immunocytochemistry of caspase-6 in MCF7 with caspase-6 antibody at 1 μg/mL.
Application
Western blot analysis of Caspase-6 in MCF7 cell lysate with Caspase-6 antibody (IN) at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of Caspase 6 in MCF7 cells with Caspase 6 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | Casp-6 antibody can be used for the detection of Caspase-6 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-6 Antibody is supplied in PBS containing 0.02% sodium azide. |
