Application
Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.Application
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.Application
Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.Application
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.Application
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.Application
Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | Caspase-8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, CASP-8 |
| Product description | Caspase-8 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP8 |
|---|---|
| Alternative Names | Caspase-8 Antibody: CAP4 MACH MCH5 FLICE ALPS2B Casp-8 Caspase-8 Apoptotic cysteine protease CASP-8 |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | 4) Cat. No. 1466 - Rat Spleen Tissue Lysate |
| Tissue Specificity | Depending on cell lines or tissues used, either full-length or other cleavage products may be observed. |
Application
Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.
Application
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.
Application
Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.
Application
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.
Application
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.
Application
Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide. |
