| Product Name | Caspase-8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease |
| Product description | Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2. |
| Immunogen | Recombinant human caspase-8 (aa 181-478). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICCIPWB |
| ICC:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP8 |
|---|---|
| Alternative Names | Apoptotic Cysteine Protease Apoptotic Protease Mch-5 CAP4 FADD-homologous ICE/ced-3-like Protease |
| Tissue Specificity | Recognizes the p18 subunit of human caspase-8. |
| Application Notes | ICC:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
