Application
Western blot analysis of lysed extracts from 293, HeLa, HepG2 and HT-29 cells using Caspase 8 Antibody.| Product Name | Caspase8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | caspase-8, zgc:92075, NCAM, CASP8, MCH5 |
| Product description | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.1) Himeji D. et. al. (2002) Blood 99:4070-4078Breckenridge D. G. et. al. (2002) Proc. Natl. Acad. Sci. U. S. A. 99:4331-4336Muzio M. et. al. (1997) J. Biol. Chem. 272:2952-2956 |
| Immunogen | Caspase8 antibody was raised against a peptide sequence around aa.221~225 (T-L-D-K-V) derived from Human Caspase8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | casp8 |
|---|---|
| Alternative Names | caspase-8 zgc:92075 NCAM CASP8 MCH5 |
| Molecular Weight(MW) | 18, 43, 57 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Caspase8 protein. |
| Entrez Gene | 58022 |
|---|---|
| Protein Accession | NP_571585.2 |
Application
Western blot analysis of lysed extracts from 293, HeLa, HepG2 and HT-29 cells using Caspase 8 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
