Application
Western Blot: Detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (Lanes 4), HEK293 (Lanes 5) and MCF7 (Lanes 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4 degrees C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.| Product Name | PTENP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTENP1This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| For ELISA 1:160000 For WB: 1:500-1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | psiPTEN Mutated in multiple advanced cancers1 antibo MMAC1 PTEN rs PTEN2 Phosphatidylinositol3 Phosphatase and tensin homolog TEP1 antibody Mutated in multiple advanced cancers 1 antibo 5 trisphosphate 3 phosphatase and dual specificity 4 5 tris |
|---|---|
| Target | PTENP1 |
| Tissue Specificity | This is directed against human PTEN-P1 protein. Reactivity occurs against human PTEN-P1 protein. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, dog, macaque, chimpanzee, rat, zebrafish sources based on 100% homology w ? ? ? ? |
Application
Western Blot: Detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (Lanes 4), HEK293 (Lanes 5) and MCF7 (Lanes 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4 degrees C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.| Application Notes | For ELISA 1:160000 For WB: 1:500-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
