Application
Immunohistochemistry of CAZIP in mouse heart tissue with CAZIP antibody at 5 μg/mL.Application
Immunofluorescence of CAZIP in mouse heart tissue with CAZIP antibody at 20 μg/mL.Application
Western blot analysis of CAZIP in A20 cell lysate with CAZIP antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CAZIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAZIP Antibody: ICIS, CAZIP, TIP150, KIAA0774, Microtubule-associated tumor suppressor candidate 2, Cardiac zipper protein, Tracking protein of 150 kDa |
| Product description | CAZIP Antibody: CAZIP, also known as TIP150, contains a leucine-zipper domain at the C-terminus and has at least three isoforms. CAZIP is expressed in heart during early cardiac development and in parts of the nervous system in later embryonic development. It is thought to bind microtubules and facilitate the end-binding protein 1 (EB1)-dependent loading of MCAK onto microtubules plus ends and orchestrates the dynamics at the plus end of microtubules. CAZIP may regulate the dynamics of microtubules at their growing distal tip.1) Du Puy L, Beqqali A, Monshouwer-Kloots J, et al. CAZIP, a novel protein expressed in the developing heart and nervous system. Dev. Dyn.2009; 238:2903-11. |
| Immunogen | CAZIP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human CAZIP. The immunogen is located within amino acids 1300 - 1350 of CAZIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CAZIP antibody can be used for detection of CAZIP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTUS2 |
|---|---|
| Alternative Names | CAZIP Antibody: ICIS CAZIP TIP150 KIAA0774 Microtubule-associated tumor suppressor candidate 2 Cardiac zipper protein Tracking protein of 150 kDa |
| Entrez Gene | 23281 |
|---|---|
| Protein Accession | NP_001028774 |
Application
Immunohistochemistry of CAZIP in mouse heart tissue with CAZIP antibody at 5 μg/mL.
Application
Immunofluorescence of CAZIP in mouse heart tissue with CAZIP antibody at 20 μg/mL.
Application
Western blot analysis of CAZIP in A20 cell lysate with CAZIP antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | CAZIP antibody can be used for detection of CAZIP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CAZIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CAZIP Antibody is supplied in PBS containing 0.02% sodium azide. |
