Application
Antibody used in WB on Human K562 at 0.2-1 ug/ml.| Product Name | CBFB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBFB, PEBP2B |
| Product description | CBFB encodes a protein that is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.1) Hart, S.M. et al., (2002) Haematologica 87 (12), 1307-1323. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CBFB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CBFB antibody can be used for detection of CBFB by ELISA at 1:312500. CBFB antibody can be used for detection of CBFB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBFB |
|---|---|
| Alternative Names | CBFB PEBP2B |
| Molecular Weight(MW) | 22 kDa, 22 kDa |
Application
Antibody used in WB on Human K562 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | CBFB antibody can be used for detection of CBFB by ELISA at 1:312500. CBFB antibody can be used for detection of CBFB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CBFB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
