Application
Confocal immunofluorescent analysis of CBFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CBFB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBFB |
| Product description | CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.1) Andersen,C.L., et.al., Br. J. Cancer 100 (3), 511-523 (2009) |
| Immunogen | This CBFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human CBFB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBFB |
|---|---|
| Alternative Names | Core-binding factor subunit beta CBF-beta Polyomavirus enhancer-binding protein 2 beta subunit PEA2-beta PEBP2-beta SL3-3 enhancer factor 1 subunit beta SL3/AKV core-binding factor beta subunit CBFB |
| Molecular Weight(MW) | 22 kDa |
Application
Confocal immunofluorescent analysis of CBFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
