Application
Western blot analysis of CBS using rabbit polyclonal CBS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CBS gene.Application
Confocal immunofluorescent analysis of CBS Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
CBS Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of CBS Antibody in mouse kidney tissue and Ramos cell line lysates (35ug/lane)| Product Name | CBS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS |
| Product description | CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.1) Ravel,C., et.al., PLoS ONE(8), E6540 (2009) |
| Immunogen | This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBS |
|---|---|
| Alternative Names | Cystathionine beta-synthase Beta-thionase Serine sulfhydrase CBS |
| Molecular Weight(MW) | 61 kDa |
Application
Western blot analysis of CBS using rabbit polyclonal CBS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CBS gene.
Application
Confocal immunofluorescent analysis of CBS Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
CBS Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of CBS Antibody in mouse kidney tissue and Ramos cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
